Limb regeneration in humans: Is it possible?

Faeeza LORGAT, Sarah GENT

Abstract


Limb regeneration is a concept that has been used in many popular movies, including the 2012 movie, The Amazing Spiderman, in which the villain, Dr. Connors, injects himself with lizard DNA with the purpose of regenerating his arm. Combining the DNA of a lizard with a human, however, will not ensure regenerative abilities in humans. This is a result of the signalling pathway, wnt signalling/ -catenin, not being activated to express regenerative genes. Natural regenerative species, such as axolotls and tadpoles, have specific Hox genes responsible for regeneration of limbs after amputation, including HoxA9, HoxA11, and HoxA13. This paper discusses the possibility of introducing these Hox genes into human DNA by virus-mediated transfection as well as using an expression vector, thereby enabling limb regeneration. The wnt signalling pathway could be used to express the three Hox genes at the wound site to stimulate regeneration of the amputated limb.


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References


Amazing Spider Man (2012) Directed by Marc Webb. [Film] USA: Colombia Pictures.

ScienceDaily (2012). Understanding how salamanders grow new limbs provides insights into potential of human regenerative medicine. Available at: https://www.sciencedaily.com/releases/2012/09/120925152139.htm [Accessed 14th March 2018].

So, V. (2014) Is Dr Conner’s Regenerative Transformation Possible? Journal of Interdisciplinary Science Topics, 3(1).

Petit, F., Sears, K. & Ahituv, N. (2017) Limb development: a paradigm of gene regulation. Nature Reviews Genetics, 18(4), pp.245-258. DOI: 10.1038/nrg.2016.167

Leander, S. (2014). How lizards regenerate their tails: researchers discover genetic 'recipe'. ASU Now: Access, Excellence, Impact. Available at: https://asunow.asu.edu/content/how-lizards-regenerate-their-tails-researchers-discover-genetic-recipe [Accessed 14th March 2018].

Kmita, M., Tarchini, B., Zàkàny, J., Logan, M., Tabin, C. & Duboule, D. (2005). Early developmental arrest of mammalian limbs lacking HoxA/HoxD gene function. Nature, 435(7045), pp.1113-1116. DOI: 10.1038/nature03648

Gardiner, D.M., Blumberg, B., Komine, Y. & Bryant, S.V. (1995). Regulation of Hox A expression in developing and regenerating axolotl limbs. Development, 121, 1731–1741.

Ohgo, S., Itoh, A., Suzuki, M., Satoh, A., Yokoyama, H. & Tamura, K. (2010). Analysis of hoxa11 and hoxa13 expression during patternless limb regeneration in Xenopus. Developmental Biology, 338(2), pp.148-157. DOI: 10.1016/j.ydbio.2009.11.026

Bastiani, M. (2013) Developmental Biology 3230. Courses.biology.utah.edu. Available at: http://courses.biology.utah.edu/bastiani/3230/DB%20Lecture/Lectures/b14Limb.html [Accessed 15th March 2018].

Wischin, S., Castañeda-Patlán, C., Robles-Flores, M. & Chimal-Monroy, J. (2017). Chemical activation of Wnt/β-catenin signalling inhibits innervation and causes skeletal tissue malformations during axolotl limb regeneration. Mechanisms of Development, 144 (B), pp 182-190. DOI: 10.1016/j.mod.2017.01.005

Kim, T.K., & Eberwine, J.H. (2010). Mammalian cell transfection: the present and the future. Analytical and Bioanalytical Chemistry, 397(8), pp 3173–3178. DOI: 10.1007/s00216-010-3821-6

VGEC (2017). Recombinant DNA and genetic techniques. Virtual Genetics Education Centre, Genetics for Higher Education, University of Leicester. Available at: https://www2.le.ac.uk/projects/vgec/highereducation/topics/recombinanttechniques [Accessed 15th March 2018].


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